pegfp n1 plasmid (TaKaRa)

TaKaRa is a verified supplier

TaKaRa manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

TaKaRa pegfp n1 plasmid
Pegfp N1 Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+n1/%E2%97%8ApHEK293+Ultra+Expression+Vector+I/pm41899473-72-5-8
Average 95 stars, based on 153 article reviews

pegfp n1 plasmid - by Bioz Stars, 2026-09

95/100 stars

Images

Polymerase Chain Reaction:

Article Title: Regulation of mitochondrial cristae organization by Myo19, Miro1 and Miro2, and metaxin 3
Article Snippet: .. Mtx2 cDNA was PCR amplified from pDEST15-MTX2 and cloned into pEGFP-N1 (Clontech) with EcoRI and BamHI. pDEST15-MTX2 was a gift from Prof. Terje Johansen (University of Tromsø, Norway). .. To create pMetaxin 3-mCherry, the full-length cDNA of human Mtx3 was amplified from Mtx3 clone BC160186 (Bio Cat, Troy, USA) by PCR and subcloned into vector pmCherry-N1 (Clontech) using restriction sites XhoI and BamHI.

Amplification:

Article Title: Molecular basis of the autoregulatory mechanism of motor neuron-related splicing factor 30
Article Snippet: DNA fragments encoding Nluc were amplified using KOD FX Neo with a primer set (KI-231/KI-232) and a DNA template (pEX-A2J1-HA-NlucP, artificial DNA synthesis service in Eurofins Genomics). .. The amplified DNA was digested with BamHI and NotI and inserted into the BamHI and NotI sites of pEGFP-N1 (Clontech). ..

Clone Assay:

Article Title: Molecular Characterization and Expression Analysis of CD22 in Nile Tilapia ( Oreochromis niloticus ) and Its Potential Role in Immune Responses.
Article Snippet: The purified PCR product and the pcDNA3.1 vector were digested with BamHI/XhoI, gel-purified, and ligated using T4 DNA ligase (TaKaRa, Kusatsu, Shiga, Japan) to generate a construct encoding His-tagged On-CD22. .. For pEGFP-OnCD22, the same CDS fragment was cloned into the BamHI/XhoI sites of pEGFP-N1 (Clontech, Mountain View, CA, USA) to obtain a C-terminal EGFP fusion plasmid. ..

Article Title: Induction of osteogenesis by delivering BMP encoding RNA
Article Snippet: .. Plasmid vectors containing codon-optimized open reading frames of Metridia luciferase (Metridia longa) and human BMP-2 mRNA were synthesized and cloned into BamHI-EcoRI sites of pVAXA120 by GeneArt (Life Technologies, CA, USA). eGFP was excised from peGFP-N1 (Clontech, CA, USA) using NotI-HindIII and cloned into EcoRI-HindIII of pVAXA120 via semi-blunt ligation. .. Coding sequence for Tomato was excised with NotI-KpnI from ptd Tomato-N1 (Clonetech, CA, USA) and ligated into EcoRI-KpnI sites of pVAXA120 via semi-blunt ligation.

Article Title: Molecular Characterization and Expression Analysis of CD22 in Nile Tilapia ( Oreochromis niloticus ) and Its Potential Role in Immune Responses
Article Snippet: The purified PCR product and the pcDNA3.1 vector were digested with BamHI/XhoI, gel-purified, and ligated using T4 DNA ligase (TaKaRa, Kusatsu, Shiga, Japan) to generate a construct encoding His-tagged On-CD22 . .. For pEGFP-OnCD22 , the same CDS fragment was cloned into the BamHI/XhoI sites of pEGFP-N1 (Clontech, Mountain View, CA, USA) to obtain a C-terminal EGFP fusion plasmid. ..

Plasmid Preparation:

Luciferase:

Synthesized:

Ligation:

Construct:

Article Title: DHX8 regulates degradation of RNA by RNautophagy
Article Snippet: These biotin-labeled oligonucleotides were synthesized by FASMAC Co. Alexa568-labeled poly-dG (15-mer) was synthesized by JBioS. .. The pCI-neo-3 × HA-biotin (HB) construct was created by subcloning synthetic DNA encoding HA 3 -Biotin (purchased from IDT) into a pCI-neo mammalian expression vector (Promega, Cat#E1841). pCI-neo-hDHX8 and pCI-neo-hDHX8-HB were constructed by subcloning the complementary DNA (cDNA) encoding human DHX8 (RIKEN BRC DNA Bank, Cat#HGY042724) into pCI-neo and pCI-neo-HB, respectively. pCI-neo-hDHX8-R620A, pCI-neo-hDHX8-R620A-HB, pCI-neo-hDHX8-NT-HB, pCI-neo-hDHX8-CT-HB, pCI-neo-hDHX8-RecA-HB, and pCI-neo-hDHX8-WRO-HB were generated by introducing a mutation into pCI-neo-hDHX8 and pCI-neo-hDHX8-HB, respectively, using the KOD-Plus-Mutagenesis Kit (TOYOBO, Cat#SMK-101). pEGFP-N1-LAMP1, pTagBFP-N-LAMP1, and pmCherry-N1-SIDT2 were constructed by subcloning cDNA encoding human LAMP1 or SIDT2 into pEGFP-N1 (Clontech, product number unavailable), pTagBFP-N (Evrogen, Cat#FP172), and pmCherry-N1 (Clontech, Cat632523), respectively. ..

Subcloning:

Expressing:

Generated:

Article Title: The N-terminal Segment of the Human ANKZF1 negatively regulates its internal mitochondrial targeting signal to prevent its mitochondrial localization
Article Snippet: .. GFP-ΔUBL-Parkin truncated variant of Parkin was generated by cloning the corresponding gene sequence at HindIII-BamHI sites of pEGFP-N1 (Clontech) vector. .. Cells were lysed by RIPA lysis buffer, protein concentration was estimated and resolved in 10-12% PAGE (polyacrylamide gel electrophoresis) followed by western transfer of proteins from gel to PVDF membrane (Millipore).

Mutagenesis:

Variant Assay:

Cloning:

Sequencing:

Affinity Purification:

Article Title: The development of a canine single-chain phage antibody library to isolate recombinant antibodies for use in translational cancer research.
Article Snippet: .. The original coding sequences were from Clontech (Clontech pEGFP-N1 and Clontech pmCherry-N1) which were subcloned into vectors with histidine and GST tags to allow for affinity purification from bacteria45 and the proteins were a gift of Professor Rory Duncan, Heriot Watt University, UK. ..

Review